Coding

Part:BBa_K1856007:Design

Designed by: Ariel Hernandez-Leyva   Group: iGEM15_Yale   (2015-09-18)


Phage Protein J0G213


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 802
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 882
    Illegal NgoMIV site found at 1015
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 451


Design Notes

This part was synthesized as a gBlock from IDT. It was cloned into backbone pSB1C3, however, we have not characterized its activity.

Source

Genomic Sequence from R.leguminosarum_Rleg9DRAFT_1750_J0GZ13. The sequence was obtained from UniProt: http://www.uniprot.org/uniprot/J0GZ13

References